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cck 8 reagent  (MedChemExpress)


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    Structured Review

    MedChemExpress cck 8 reagent
    Cck 8 Reagent, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 4576 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+counting+kit+8+cck+8+reagent/Cell+Counting+Kit-8/10__1016_slash_j__apsb__2026__08__007-107-17-19
    Average 99 stars, based on 4576 article reviews
    cck 8 reagent - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Cell Attachment Assay:

    Article Title: Circular RNA hsa_Circ_0007552 inhibits lung adenocarcinoma proliferation, migration and invasion via the miR-7974/BAP1 axis
    Article Snippet: .. After cell attachment, cell proliferation viability was examined using cell counting kit-8 (CCK-8) reagent (MCE, MedChemExpress, shanghai, China) once every 24h for 5 days. ..

    CCK-8 Assay:

    Article Title: Circular RNA hsa_Circ_0007552 inhibits lung adenocarcinoma proliferation, migration and invasion via the miR-7974/BAP1 axis
    Article Snippet: .. After cell attachment, cell proliferation viability was examined using cell counting kit-8 (CCK-8) reagent (MCE, MedChemExpress, shanghai, China) once every 24h for 5 days. ..

    Article Title: TIMELESS Promotes LUAD Growth via Suppressing Transferrin-Mediated Ferroptosis and Reprograms the Tumor Microenvironment against Anti-PD-1 Immunotherapy
    Article Snippet: Erastin (MedChemExpress, Cat. HY-15763) or RSL3 (MedChemExpress, Cat. HY-100218A) was then added to the wells at various concentrations (0, 0.1, 1, 3, 5, and 10 μmol/l) for 48 h. After incubation, the culture medium was discarded. .. Cell counting kit-8 (CCK-8) reagent (MedChemExpress, Cat. HY-K0301) was added to each well, and the absorbance at 450 nm was measured using a microplate reader (TECAN, Infinite 200 PRO). ..

    Article Title: Exploring the mechanism of piperlongumine in promoting ferroptosis in cervical cancer based on network pharmacology and molecular docking analysis
    Article Snippet: Once the SiHa and C-33A cells reached a stable growth phase, they were randomly assigned into four distinct experimental groups and subsequently exposed to different concentrations (0, 2, 4, 6, and 8 μM) of piperlongumine (MedChemExpress) for 48 h. .. SiHa and C-33A cells were seeded into a 96-well plate at a density of 3000 cells/well and subjected to piperlongumine treatment for 48 h. Then, each well was supplemented with 10 μL of cell counting kit-8 (CCK-8) reagent (MedChemExpress) along with 90 μL of fresh complete medium. ..

    Article Title: Growth differentiation factor 7 alleviates the proliferation and metastasis of hepatocellular carcinoma
    Article Snippet: .. At the indicated time points, the cells were incubated with 10 μL/well of cell counting kit-8 (CCK-8) reagent (#HY-K0301, MedChem Express, Monmouth Junction, NJ, USA). .. After 2 h of incubation, optical density (OD) values were determined using a microplate reader (BioTek, Winooski, VT, USA) at a wavelength of 450 nm.

    Article Title: Growth differentiation factor 7 alleviates the proliferation and metastasis of hepatocellular carcinoma
    Article Snippet: .. At the indicated time points, the cells were incubated with 10 μL/well of cell counting kit-8 (CCK-8) reagent (#HY-K0301, MedChem Express, Monmouth Junction, NJ, USA). .. After 2 h of incubation, optical density (OD) values were determined using a microplate reader (BioTek, Winooski, VT, USA) at a wavelength of 450 nm.

    Article Title: Rapid and high-throughput screening of proteolysis targeting chimeras using a dual-reporter system expressing fluorescence protein and luciferase
    Article Snippet: .. Thereafter, each well received 100 μL of media with 10% cell counting kit-8 (CCK-8) reagent (MCE, HY-K0301, NJ, USA), and the plates were incubated for 2 h at 37 °C in an incubator with 5% CO 2 . ..

    Article Title: Rapid and high-throughput screening of proteolysis targeting chimeras using a dual-reporter system expressing fluorescence protein and luciferase.
    Article Snippet: .. Thereafter, each well received 100 μL of media with 10% cell counting kit-8 (CCK-8) reagent (MCE, HY-K0301, NJ, USA), and the plates were incubated for 2 h at 37 °C in an incubator with 5% CO2. ..

    Incubation:

    Article Title: Growth differentiation factor 7 alleviates the proliferation and metastasis of hepatocellular carcinoma
    Article Snippet: .. At the indicated time points, the cells were incubated with 10 μL/well of cell counting kit-8 (CCK-8) reagent (#HY-K0301, MedChem Express, Monmouth Junction, NJ, USA). .. After 2 h of incubation, optical density (OD) values were determined using a microplate reader (BioTek, Winooski, VT, USA) at a wavelength of 450 nm.

    Article Title: Growth differentiation factor 7 alleviates the proliferation and metastasis of hepatocellular carcinoma
    Article Snippet: .. At the indicated time points, the cells were incubated with 10 μL/well of cell counting kit-8 (CCK-8) reagent (#HY-K0301, MedChem Express, Monmouth Junction, NJ, USA). .. After 2 h of incubation, optical density (OD) values were determined using a microplate reader (BioTek, Winooski, VT, USA) at a wavelength of 450 nm.

    Article Title: Rapid and high-throughput screening of proteolysis targeting chimeras using a dual-reporter system expressing fluorescence protein and luciferase
    Article Snippet: .. Thereafter, each well received 100 μL of media with 10% cell counting kit-8 (CCK-8) reagent (MCE, HY-K0301, NJ, USA), and the plates were incubated for 2 h at 37 °C in an incubator with 5% CO 2 . ..

    Article Title: Rapid and high-throughput screening of proteolysis targeting chimeras using a dual-reporter system expressing fluorescence protein and luciferase.
    Article Snippet: .. Thereafter, each well received 100 μL of media with 10% cell counting kit-8 (CCK-8) reagent (MCE, HY-K0301, NJ, USA), and the plates were incubated for 2 h at 37 °C in an incubator with 5% CO2. ..



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    Dojindo Labs cck 8 reagent
    PLXNC1 promotes the proliferation, migration, and invasion of colorectal cancer (CRC) cells in vitro. (A) qRT-PCR analysis was conducted to examine the knockdown efficiency of PLXNC1 in the negative control (NC)-siRNA- or PLXNC1-siRNA-transfected CRC cells. NC-siRNA was used as a negative control. GAPDH was a normalization control. <t>(B)</t> <t>CCK-8</t> assay was performed to assay the viability of control and PLXNC1-silenced CRC cells. (C) After 48 h of transfection with NC-siRNA or PLXNC1-siRNA, the migration of CRC cells in Transwell chambers was determined. (D) After 48 h of transfection with NC-siRNA or PLXNC1-siRNA, CRC cells invading the Matrigel in Transwell chambers were performed. Note: Scale bars = 200 μm. (E) Representative images of EdU-stained (red) cells and the quantification of cell proliferation ratio normalized to Hoechst-stained (blue) cells. Scale bars = 75 μm. (F) qRT-PCR data of the PLXNC1 expression in LoVo cells following transfection with PLXNC1 plasmids or controls. GAPDH was a normalization control. (G) CCK-8 assay results show the viability of LoVo cells following transfection with PLXNC1 plasmids or controls. (H, I) Transwell assay was employed to count the number of migrated (H) and invaded (I) LoVo cells following transfection with PLXNC1 plasmids or controls. ∗∗∗ p < 0.001; ∗∗ p < 0.01, and ∗ p < 0.05.
    Cck 8 Reagent, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+counting+kit+8+cck+8+reagent/Cell+Counting+Kit-8/pmc13090551-93-0-4
    Average 99 stars, based on 1 article reviews
    cck 8 reagent - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    99
    MedChemExpress cck 8 reagent
    PLXNC1 promotes the proliferation, migration, and invasion of colorectal cancer (CRC) cells in vitro. (A) qRT-PCR analysis was conducted to examine the knockdown efficiency of PLXNC1 in the negative control (NC)-siRNA- or PLXNC1-siRNA-transfected CRC cells. NC-siRNA was used as a negative control. GAPDH was a normalization control. <t>(B)</t> <t>CCK-8</t> assay was performed to assay the viability of control and PLXNC1-silenced CRC cells. (C) After 48 h of transfection with NC-siRNA or PLXNC1-siRNA, the migration of CRC cells in Transwell chambers was determined. (D) After 48 h of transfection with NC-siRNA or PLXNC1-siRNA, CRC cells invading the Matrigel in Transwell chambers were performed. Note: Scale bars = 200 μm. (E) Representative images of EdU-stained (red) cells and the quantification of cell proliferation ratio normalized to Hoechst-stained (blue) cells. Scale bars = 75 μm. (F) qRT-PCR data of the PLXNC1 expression in LoVo cells following transfection with PLXNC1 plasmids or controls. GAPDH was a normalization control. (G) CCK-8 assay results show the viability of LoVo cells following transfection with PLXNC1 plasmids or controls. (H, I) Transwell assay was employed to count the number of migrated (H) and invaded (I) LoVo cells following transfection with PLXNC1 plasmids or controls. ∗∗∗ p < 0.001; ∗∗ p < 0.01, and ∗ p < 0.05.
    Cck 8 Reagent, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+counting+kit+8+cck+8+reagent/Cell+Counting+Kit-8/10__1016_slash_j__apsb__2026__08__007-107-17-19
    Average 99 stars, based on 1 article reviews
    cck 8 reagent - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    86
    Yeasen Biotechnology cell count kit 8 cck 8 reagent
    PLXNC1 promotes the proliferation, migration, and invasion of colorectal cancer (CRC) cells in vitro. (A) qRT-PCR analysis was conducted to examine the knockdown efficiency of PLXNC1 in the negative control (NC)-siRNA- or PLXNC1-siRNA-transfected CRC cells. NC-siRNA was used as a negative control. GAPDH was a normalization control. <t>(B)</t> <t>CCK-8</t> assay was performed to assay the viability of control and PLXNC1-silenced CRC cells. (C) After 48 h of transfection with NC-siRNA or PLXNC1-siRNA, the migration of CRC cells in Transwell chambers was determined. (D) After 48 h of transfection with NC-siRNA or PLXNC1-siRNA, CRC cells invading the Matrigel in Transwell chambers were performed. Note: Scale bars = 200 μm. (E) Representative images of EdU-stained (red) cells and the quantification of cell proliferation ratio normalized to Hoechst-stained (blue) cells. Scale bars = 75 μm. (F) qRT-PCR data of the PLXNC1 expression in LoVo cells following transfection with PLXNC1 plasmids or controls. GAPDH was a normalization control. (G) CCK-8 assay results show the viability of LoVo cells following transfection with PLXNC1 plasmids or controls. (H, I) Transwell assay was employed to count the number of migrated (H) and invaded (I) LoVo cells following transfection with PLXNC1 plasmids or controls. ∗∗∗ p < 0.001; ∗∗ p < 0.01, and ∗ p < 0.05.
    Cell Count Kit 8 Cck 8 Reagent, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+counting+kit+8+cck+8+reagent/8+cck/pm42310756-40-9-16
    Average 86 stars, based on 1 article reviews
    cell count kit 8 cck 8 reagent - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    Image Search Results


    PLXNC1 promotes the proliferation, migration, and invasion of colorectal cancer (CRC) cells in vitro. (A) qRT-PCR analysis was conducted to examine the knockdown efficiency of PLXNC1 in the negative control (NC)-siRNA- or PLXNC1-siRNA-transfected CRC cells. NC-siRNA was used as a negative control. GAPDH was a normalization control. (B) CCK-8 assay was performed to assay the viability of control and PLXNC1-silenced CRC cells. (C) After 48 h of transfection with NC-siRNA or PLXNC1-siRNA, the migration of CRC cells in Transwell chambers was determined. (D) After 48 h of transfection with NC-siRNA or PLXNC1-siRNA, CRC cells invading the Matrigel in Transwell chambers were performed. Note: Scale bars = 200 μm. (E) Representative images of EdU-stained (red) cells and the quantification of cell proliferation ratio normalized to Hoechst-stained (blue) cells. Scale bars = 75 μm. (F) qRT-PCR data of the PLXNC1 expression in LoVo cells following transfection with PLXNC1 plasmids or controls. GAPDH was a normalization control. (G) CCK-8 assay results show the viability of LoVo cells following transfection with PLXNC1 plasmids or controls. (H, I) Transwell assay was employed to count the number of migrated (H) and invaded (I) LoVo cells following transfection with PLXNC1 plasmids or controls. ∗∗∗ p < 0.001; ∗∗ p < 0.01, and ∗ p < 0.05.

    Journal: Genes & Diseases

    Article Title: Identification of PLXNC1 as a novel biomarker for consensus molecular subtype 4 in colorectal cancer

    doi: 10.1016/j.gendis.2025.101974

    Figure Lengend Snippet: PLXNC1 promotes the proliferation, migration, and invasion of colorectal cancer (CRC) cells in vitro. (A) qRT-PCR analysis was conducted to examine the knockdown efficiency of PLXNC1 in the negative control (NC)-siRNA- or PLXNC1-siRNA-transfected CRC cells. NC-siRNA was used as a negative control. GAPDH was a normalization control. (B) CCK-8 assay was performed to assay the viability of control and PLXNC1-silenced CRC cells. (C) After 48 h of transfection with NC-siRNA or PLXNC1-siRNA, the migration of CRC cells in Transwell chambers was determined. (D) After 48 h of transfection with NC-siRNA or PLXNC1-siRNA, CRC cells invading the Matrigel in Transwell chambers were performed. Note: Scale bars = 200 μm. (E) Representative images of EdU-stained (red) cells and the quantification of cell proliferation ratio normalized to Hoechst-stained (blue) cells. Scale bars = 75 μm. (F) qRT-PCR data of the PLXNC1 expression in LoVo cells following transfection with PLXNC1 plasmids or controls. GAPDH was a normalization control. (G) CCK-8 assay results show the viability of LoVo cells following transfection with PLXNC1 plasmids or controls. (H, I) Transwell assay was employed to count the number of migrated (H) and invaded (I) LoVo cells following transfection with PLXNC1 plasmids or controls. ∗∗∗ p < 0.001; ∗∗ p < 0.01, and ∗ p < 0.05.

    Article Snippet: CCK-8 reagent (10 μL, DOJINDO, Kumamoto, Japan) was added to each well at 0, 24, 48, and 72 h after transfection and incubated at 37 °C for 4 h. Absorbance at 450 nm was measured using a microplate reader (PerkinElmer EnVision, Massachusetts, USA).

    Techniques: Migration, In Vitro, Quantitative RT-PCR, Knockdown, Negative Control, Transfection, Control, CCK-8 Assay, Staining, Expressing, Transwell Assay